Department: Blizard Institute
Salary: £36,677- £41,991 per annum (Grade 5)
Date posted: 19-Dec-2017
Closing date: 20-Jan-2018
Modelling of Alzheimer’s Disease in human induced pluripotency stem cells (hiPSC) using trisomy 21 (Down Syndrome) as extreme phenotype approach
We are looking for postdoctoral research assistant with a track record of accomplishment (either during the PhD studentship, or during a previous postdoctoral research) demonstrating ability in the field of cell biology. Experience with cell culture experimentation, and characterization of cell phenotypes are desirable, especially experience in work with human embyonic stem cells or iPSC would be an advantage.
Down syndrome (DS), caused by trisomy 21 (T21), has an incidence of ~1/650 live births, with an increasing trend. Pathological components of DS (frequently common diseases in general population, such as Alzheimer’s dementia, childhood leukaemia and others) have correlates measurable as in vitro phenotypes in T21 cells, and our lab is leading internationally in the establishment of these cellular phenotypes, and their correction using small chemicals (Murray et al. Stem Cells 2015, Wiseman et al. Nature Rev. Neuroscience 2015, Nikolaev et al. Nature Communications 2015, Nizetic & Groet Nature Rev. Cancer 2012, Canzonetta et al. AJHG 2008, De Vita et al. Oncogene 2010, Reynolds et al. Nature 2010, Wang et al. Mol Cell Proteomics 2009). We recently developed a unique isogenic human induced-pluripotency-stem-cells (hiPSC) model by non-integration re-programming of skin fibroblasts from a mosaic DS individual, and then cloning separately the genotypically identical T21 and euploid iPSC lines. The postdoctoral reasearcher will lead the project using the isogenic hiPSC system to model the cellular phenotypes we recently established, to “distil” the pure effects of T21, devoid of genotypic and artificial variation factors, and substantially improve our understanding of the pathomechanisms of DS and Alzheimer’s dementia, and the design of hi-throughput chemical screening assays for phenotype correction. She/He will also be tought the skills of generation of cerebral organoids and 3D neuronal cultures, and have access to additional hiPSC from patients clinically stratified for Alzhemer’s dementia. In addition, a range of genetic models of Alzheimer’s dementia (partial trisomies and CRSPR-reduced single gene trisomies) will be available. The postdoc will lead on the cellular part of the Wellcome Trust funded “LonDownS Consortium” project, and also the newly starting project funded by the EU-Joint Pathways in Neurodegeneration.
The lab (http://www.icms.qmul.ac.uk/Profiles/Paediatrics/Nizetic%20Dean.htm) is located within the modern, futuristic Blizard building, attached to the Royal London Hospital campus. The Blizard institute is a 400 researcher’s strong hub of cell and molecular science, and is equipped with the latest state-of-art FACS and high-content and high-throughput confocal microscopy equipment manned core facilities.
The post is full time initially for 12 months, with a possibility of extension for a further 18 months. Starting salary will be in the range £36,677–£41,991 per annum depending on prior accomplishment and experience inclusive of London Allowance. Benefits include 30 days annual leave, defined benefit pension scheme and interest-free season ticket loan.
Candidates must be able to demonstrate their eligibility to work in the UK in accordance with the Immigration, Asylum and Nationality Act 2006. Where required this may include entry clearance or continued leave to remain under the Points Based Immigration Scheme.
Please send your C.V, including the list of publications, and contact details (including telephone numbers) of referees and specific questions about the project should be addressed to Professor Dean Nizetic (firstname.lastname@example.org) or 0207 882 2275.
Details about the Blizard Institute can be found at www.blizard.qmul.ac.uk.
Application enquiries should be directed to email@example.com.
To apply, please click on the apply link below.
The closing date for the applications is 20th January 2018.
Interviews with remotely located candidates could be organized via FaceTime or Skype. A successful candidate is expected to start in the first week of February 2018.
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